|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8610||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2641
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namebarstar A
Insert Size (bp)439
MutationTwo cysteines of barstar changed to alanine, C40A and C82A
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
As pMT316 but with the TGT codons for the two cysteines of barstar, C40 and C82, changed to GCG for alanine. The protein product, barstar A, has no sulfhydryl groups but is functional in vivo and in vitro, with only slight reductions in barnase binding and stability.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMT643 was a gift from Robert Hartley (Addgene plasmid # 8610 ; http://n2t.net/addgene:8610 ; RRID:Addgene_8610)
For your References section:Directed mutagenesis and barnase-barstar recognition. Hartley RW. Biochemistry 1993 Jun 15;32(23):5978-84. 10.1021/bi00074a008 PubMed 8507637