PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86391||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4779
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameD. melanogaster zfh1 enhancer
SpeciesD. melanogaster (fly)
Insert Size (bp)862
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3_zfh1_CP-candidate_luc+ was a gift from Alexander Stark (Addgene plasmid # 86391 ; http://n2t.net/addgene:86391 ; RRID:Addgene_86391)
For your References section:Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution. Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, Stark A. Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739. 10.1038/nbt.3739 PubMed 28024147