pFH6
(Plasmid #86555)

• Purpose: Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency
• Depositing Lab: Andreas Weber
• Publication: Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017.

Backbone

• Vector backbone: pGEM T-easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3017
• Total vector size (bp): 3620
• Vector type: CRISPR ; Subcloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6-26p::sgRNA scaffold
• gRNA/shRNA sequence: can be inserted via BbsI sites
• Species: A. thaliana (mustard weed), Synthetic
• Promoter: Arabidopsis U6-26 promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gtaaaacgacggccag
• 3′ sequencing primer: caggaaacagctatgac

Resource Information

• Supplemental Documents:
  • CRISPR Protocol
• A portion of this plasmid was derived from a plasmid made by: Peter Hegemann (HU Berlin)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See Hahn et al., 2017