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An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana.
Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP
Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017. PubMed Article

Plasmids from Article

ID Plasmid Purpose
86555pFH6Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency
86556pUB-Cas9For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
86779pUB-Cas9-@GL1Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

Antibodies from Article