pA5c-tesA-vhb50-8fadR
(Plasmid #86601)

• Purpose: expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter
• Depositing Lab: Yinjie Tang
• Publication: Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1002/bit.26067.

Backbone

• Vector backbone: unknown
• Total vector size (bp): 5123
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: tesA
• Alt name: encoding enzyme for free fatty acid biosynthesis
• Promoter: LacUV5

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Lac-promoter

Gene/Insert 2

• Gene/Insert name: Vhb50
• Alt name: encoding Vitreoscilla hemoglobin
• Promoter: LacUV5

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Lac-promoter
• 3′ sequencing primer: rrnB-T1-term-Rev

Gene/Insert 3

• Gene/Insert name: fadR
• Alt name: E coli fatty acid pathway regulator
• Species: E. coli
• Promoter: pBAD

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: pBAD-F

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry