pRB2960
(Plasmid #8677)

• Depositing Lab: David Botstein
• Publication: Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

Backbone

• Vector backbone: pTS408
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: Don't know
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP::TUB1 under GAL1 promoter
• Alt name: TUB1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: TUB1 (a.k.a. YML085C)
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See comment (unknown if destroyed)
• 3′ cloning site: See comment (unknown if destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TUB1 wildtype DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.