pBluescript-mouse cyclin D2
(Plasmid #86791)

• Purpose: bacterial expression of mouse cyclin D2
• Depositing Labs: Martine Roussel, Charles Sherr
• Publication: Matsushime et al Cell. 1991 May 17;65(4):701-13.

Backbone

• Vector backbone: pBS II KS+
• Backbone size w/o insert (bp): 1200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin D2
• Alt name: ccnd2
• Species: M. musculus (mouse)
• Entrez Gene: Ccnd2 (a.k.a. 2600016F06Rik, Vin-1, Vin1, cD2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Matsushime when in Drs Sherr and Roussel laboratories

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry