|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8680||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)995
Mutationmakes protein by Coomassie blue staining
Entrez GeneHOXC13 (a.k.a. ECTD9, HOX3, HOX3G)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byAlexander Awgulewitsch
Terms and Licenses
Tkatchenko et al Development 128: 1547-1558 (2001)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXC13(human) GSTfusion-pET was a gift from Corey Largman (Addgene plasmid # 8680 ; http://n2t.net/addgene:8680 ; RRID:Addgene_8680)
For your References section:HOXB13 homeodomain protein is cytoplasmic throughout fetal skin development. Komuves LG, Ma XK, Stelnicki E, Rozenfeld S, Oda Y, Largman C. Dev Dyn 2003 Jun;227(2):192-202. 10.1002/dvdy.10290 PubMed 12761847
Map uploaded by the depositor.