pCDNA3.1 (-) + codon optimized Brn3a
Purposemammalian expression of the transcription factor Brn3a
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86829||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCDNA3.1 (-)
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePOU4F1 (a.k.a. BRN3A, Oct-T1, RDC-1, brn-3A)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHR (Common Sequencing Primers)
The wild-type sequence of Brn3a was codon optimized to allow for gene synthesis of gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3.1 (-) + codon optimized Brn3a was a gift from Carl Novina (Addgene plasmid # 86829 ; http://n2t.net/addgene:86829 ; RRID:Addgene_86829)
For your References section:The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon CH, Novina CD. Cell Rep. 2016 May 31;15(9):2025-37. doi: 10.1016/j.celrep.2016.04.018. Epub 2016 May 19. 10.1016/j.celrep.2016.04.018 PubMed 27210747