Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pXP420 v2 (repaired Amp promoter)
(Plasmid #86920)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86920 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    Sandmeyer lab (Addgene plasmid 26844)
  • Modifications to backbone
    Ampicillin promoter was corrected to repair a split in the promoter that occurred during the original construction of pX420 using the Ssp1 site for cloning, as described in Fang et al., Yeast. 2011 Feb;28(2):123-36.
  • Vector type
    Yeast Expression, Cre/Lox
  • Promoter TEF1
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer TEF_seq (5'-ggtcttcaatttctcaagtttc-3')
  • 3′ sequencing primer CYC1 (5'-GCGTGAATGTAAGCGTGAC-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the corrected Ampicillin promoter may improve the bacterial resistance of the plasmid to ampicillin compared to the original pXP420 (Addgene plasmid# 26844).

The HIS3 selection marker in this plasmid contains a V132A mutation in imidazoleglycerol-phosphate dehydratase. Yeast transformants grow normally on SC-HIS plates, so this mutation does not inhibit gene function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXP420 v2 (repaired Amp promoter) was a gift from Gary Yellen (Addgene plasmid # 86920 ; http://n2t.net/addgene:86920 ; RRID:Addgene_86920)