pcDNA3 mRuby2 LIC cloning vector (6H)
Purpose(Empty Backbone) Empty LIC vector; adds an mRuby2 gene to the C-terminus of your protein of interest.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86965||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6139
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Protein
- mRuby2 (C terminal on backbone)
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV F (5'cgcaaatgggcggtaggcgtg) (Common Sequencing Primers)
This is an empty LIC vector derived from pcDNA3. It adds an mRuby2 gene to the C-terminus of your protein of interest.
To clone into this vector, add the following tags to your PCR primers:
LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)
LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC
Do NOT include a stop codon with your reverse primer.
T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.
For more information, please see our website:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 mRuby2 LIC cloning vector (6H) was a gift from Chris Jeans (Addgene plasmid # 86965 ; http://n2t.net/addgene:86965 ; RRID:Addgene_86965)