|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8699||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10200
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. melanogaster (fly)
Insert Size (bp)320
Entrez GeneHis4:CG31611 (a.k.a. Dmel_CG31611, CG31611, Dmel\CG31611)
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EagI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer CCATCTAATTCAACAAGAATTGGG ACAAC (Common Sequencing Primers)
Terms and Licenses
Heat shock promoter. Ahmad lab #k163.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HS-H4-GFP was a gift from Kami Ahmad (Addgene plasmid # 8699 ; http://n2t.net/addgene:8699 ; RRID:Addgene_8699)
For your References section:The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. Ahmad K, Henikoff S. Mol Cell 2002 Jun;9(6):1191-200. 10.1016/S1097-2765(02)00542-7 PubMed 12086617