pET28b-pvdA WT
(Plasmid #87098)

• Purpose: expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa
• Depositing Lab: Audrey Lamb
• Publication: Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98. doi: 10.1074/jbc.M111.265876. Epub 2011 Jul 13.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5368
• Total vector size (bp): 6700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 3-4 hours growth after induction
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pvdA WT
• Species: Pseudomonas aeruginosa PAO1
• Insert Size (bp): 1332
• Promoter: T7
• Tag / Fusion Protein:
  • his-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pet upstream
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Biochemistry, 2007, 46, 11930-11937, PMC2597334
Biochemistry 2009, 48, 4371-4376, PMC2710847