pET28-OmpT
(Plasmid #68862)

• Purpose: construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.
• Depositing Lab: Neil Kelleher
• Publication: Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi: 10.1038/nmeth.2074.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OmpT
• Alt name: Protease 7
• Alt name: Omptin Outer membrane protein 3B
• Alt name: Protease A, Protease VII
• GenBank ID: NC_000913.3
• Entrez Gene: ompT (a.k.a. b0565, ECK0557)
• Tag / Fusion Protein:
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ATTAATCCATGGCTTC TCGAGACTTTATCGTTTA
• 3′ sequencing primer: ACTCGGGAATTCTT AAAAGTGTACTTAAGACCAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cong Wu
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry