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pKT0209
(Plasmid #8730)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8730 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a-link
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Yeast Expression
  • Selectable markers
    C. albicans URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yEGFP
  • Alt name
    Codon-optimized GFP
  • Tag / Fusion Protein
    • Codon optimized linker (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Full name of this plasmid is pFA6a-link-yEGFP-CaURA3. An codon-optimized linker
was added into the yEGFP to improve expression level. C.
albicans URA3 (CaURA3) was introduced by subcloning the BglII/SacI
fragment of pAG60 (Goldstein et al., 1999) into the plasmid, in place
of SpHIS5.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT0209 was a gift from Kurt Thorn (Addgene plasmid # 8730)
  • For your References section:

    Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731