|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8730||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeYeast Expression
Selectable markersC. albicans URA3
Growth in Bacteria
Alt nameCodon-optimized GFP
/ Fusion Protein
- Codon optimized linker (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Full name of this plasmid is pFA6a-link-yEGFP-CaURA3. An codon-optimized linker
was added into the yEGFP to improve expression level. C.
albicans URA3 (CaURA3) was introduced by subcloning the BglII/SacI
fragment of pAG60 (Goldstein et al., 1999) into the plasmid, in place
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKT0209 was a gift from Kurt Thorn (Addgene plasmid # 8730)
For your References section:Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731