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Addgene

3158 pSFFV-neo Bad cDNA
(Plasmid #8753)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8753 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    615
  • GenBank ID
    NM_007522
  • Entrez Gene
    Bad (a.k.a. AI325008, Bbc2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gabriel Nunez

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The expression plasmid pSFFVHA-Bad was constructed by using PCR primers, which incorporated the 9 codons of the HA epitope onto the 5' end of Bad, and it was ligated into pSFFV vector at the EcoRI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3158 pSFFV-neo Bad cDNA was a gift from Stanley Korsmeyer (Addgene plasmid # 8753 ; http://n2t.net/addgene:8753 ; RRID:Addgene_8753)
  • For your References section:

    Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Yang E, Zha J, Jockel J, Boise LH, Thompson CB, Korsmeyer SJ. Cell 1995 Jan 27;80(2):285-91. 10.1016/0092-8674(95)90411-5 PubMed 7834748