pHmK-C1
(Plasmid
#87866)
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PurposeBackbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
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Depositing Lab
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Depositing OrganizationJuntendo University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 87866-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 87866-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerCLONTECH
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5444
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Modifications to backboneThe DNA NheI-BspEI fragment of EGFP within pEGFP-C1 was replaced to the DNA NheI-BspEI fragment of pHluorin-mKate2 (PK) probe.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepHluorin-mKate2 fusion
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Alt namePK-tag
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Insert Size (bp)1500
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GenBank IDAF058695.1 KJ908191.1
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Tag
/ Fusion Protein
- pHluorin-mKate2 fusion (C terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe cDNA of pHluroin was kindly given from Dr. James Rothman.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The DNA NheI-BspEI fragment of EGFP within pEGFP-C1 was replaced to the DNA NheI-BspEI fragment of pHluorin-mKate2 (PK) probe.
DNA (Catalog # 87866-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 87866-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHmK-C1 was a gift from Isei Tanida (Addgene plasmid # 87866 ; http://n2t.net/addgene:87866 ; RRID:Addgene_87866) -
For your References section:
A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. Tanida I, Ueno T, Uchiyama Y. PLoS One. 2014 Oct 23;9(10):e110600. doi: 10.1371/journal.pone.0110600. eCollection 2014. 10.1371/journal.pone.0110600 PubMed 25340751
