pHmK-C1
(Plasmid #87866)

• Purpose: Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
• Depositing Lab: Isei Tanida
• Publication: Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi: 10.1371/journal.pone.0110600. eCollection 2014.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: CLONTECH
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5444
• Modifications to backbone: The DNA NheI-BspEI fragment of EGFP within pEGFP-C1 was replaced to the DNA NheI-BspEI fragment of pHluorin-mKate2 (PK) probe.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pHluorin-mKate2 fusion
• Alt name: PK-tag
• Insert Size (bp): 1500
• GenBank ID: AF058695.1 KJ908191.1
• Tag / Fusion Protein:
  • pHluorin-mKate2 fusion (C terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cDNA of pHluroin was kindly given from Dr. James Rothman.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DNA NheI-BspEI fragment of EGFP within pEGFP-C1 was replaced to the DNA NheI-BspEI fragment of pHluorin-mKate2 (PK) probe.