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GAL-hAR-658-919
(Plasmid #89082)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89082 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hAR-658-919
  • Species
    H. sapiens (human)
  • Mutation
    aa 658-919
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Tag / Fusion Protein
    • GAL4 DBD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site TthIII (unknown if destroyed)
  • 3′ cloning site Csp45I (unknown if destroyed)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This vector contains a short VP16 activation domain (AA 411-456.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAL-hAR-658-919 was a gift from Elizabeth Wilson (Addgene plasmid # 89082 ; http://n2t.net/addgene:89082 ; RRID:Addgene_89082)
  • For your References section:

    Modulation of androgen receptor activation function 2 by testosterone and dihydrotestosterone. Askew EB, Gampe RT Jr, Stanley TB, Faggart JL, Wilson EM. J Biol Chem. 2007 Aug 31;282(35):25801-16. doi: 10.1074/jbc.M703268200. Epub 2007 Jun 25. 10.1074/jbc.M703268200 PubMed 17591767