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PurposeExpression of hDicer in Sf9 Cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastBac
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Backbone manufacturerThermo Fisher Scientific
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehDicer
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SpeciesH. sapiens (human)
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Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
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Tag
/ Fusion Protein
- His6-tev (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac-hDicer was a gift from Jennifer Doudna (Addgene plasmid # 89144 ; http://n2t.net/addgene:89144 ; RRID:Addgene_89144) -
For your References section:
In vitro reconstitution of the human RISC-loading complex. MacRae IJ, Ma E, Zhou M, Robinson CV, Doudna JA. Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):512-7. doi: 10.1073/pnas.0710869105. Epub 2008 Jan 4. 10.1073/pnas.0710869105 PubMed 18178619
