Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #162162)


Item Catalog # Description Quantity Price (USD)
Plasmid 162162 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Drosophila Genomics Resource Center
  • Total vector size (bp) 8827
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    Synthetic; ; S. pyogenes
  • Insert Size (bp)
  • Promoter Ae. aegypti PUb
  • Tag / Fusion Protein
    • N/A

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter Ae. aegypti U6

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Peter Duchek, Addgene, pDCC6

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKRG3-mU6-PUb-hSpCas9 was a gift from Charles Rice (Addgene plasmid # 162162 ; ; RRID:Addgene_162162)
  • For your References section:

    A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Rozen-Gagnon K, Yi S, Jacobson E, Novack S, Rice CM. Sci Rep. 2021 Jan 12;11(1):736. doi: 10.1038/s41598-020-80436-5. 10.1038/s41598-020-80436-5 PubMed 33436886