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A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines.
Rozen-Gagnon K, Yi S, Jacobson E, Novack S, Rice CM
Sci Rep. 2021 Jan 12;11(1):736. doi: 10.1038/s41598-020-80436-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
162161pKRG3-mU6-PUb-3xFLAG-hSpCas9Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)
162162pKRG3-mU6-PUb-hSpCas9Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)
162163pKRG3-mU6-PUb-hSpCas9-pAcExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable
162164pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1Selectable homology donor template for Aag2 cell AGO1

Antibodies from Article