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pKRG3-mU6-PUb-hSpCas9-pAc
(Plasmid #162163)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162163 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHW
  • Backbone manufacturer
    Drosophila Genomics Resource Center
  • Total vector size (bp) 9532
  • Vector type
    Insect Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hSpCas9
  • Species
    Synthetic; ; S. pyogenes
  • Insert Size (bp)
    4890
  • Promoter Ae. aegypti PUb
  • Tag / Fusion Protein
    • T2A-pAc (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    193
  • Promoter Ae. aegypti U6

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Peter Duchek, Addgene, pDCC6
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKRG3-mU6-PUb-hSpCas9-pAc was a gift from Charles Rice (Addgene plasmid # 162163 ; http://n2t.net/addgene:162163 ; RRID:Addgene_162163)
  • For your References section:

    A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Rozen-Gagnon K, Yi S, Jacobson E, Novack S, Rice CM. Sci Rep. 2021 Jan 12;11(1):736. doi: 10.1038/s41598-020-80436-5. 10.1038/s41598-020-80436-5 PubMed 33436886