pBMN HA-GBRP
(Plasmid
#89296)
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PurposeFor expression of the mammalian Atg8 protein GABARAP tagged with HA
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBMN
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGABARAP
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Alt nameMM46
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Alt nameATG8A
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SpeciesH. sapiens (human)
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Entrez GeneGABARAP (a.k.a. ATG8A, GABARAP-a, MM46)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBMN 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN HA-GBRP was a gift from Michael Lazarou (Addgene plasmid # 89296 ; http://n2t.net/addgene:89296 ; RRID:Addgene_89296) -
For your References section:
Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M. J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18. 10.1083/jcb.201607039 PubMed 27864321