pMX-IY HA-GBRPL1
(Plasmid #89297)

• Purpose: For expression of the mammalian Atg8 protein GABARAPL1 tagged with HA
• Depositing Lab: Michael Lazarou
• Publication: Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.

Backbone

• Vector backbone: pMX
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: IRES-YFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABARAPL1
• Alt name: GEC-1
• Alt name: ATG8L
• Species: H. sapiens (human)
• Entrez Gene: GABARAPL1 (a.k.a. APG8-LIKE, APG8L, ATG8, ATG8B, ATG8L, GEC1)
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • IRES-YFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBMN 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry