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Addgene

LMP AMPK alpha2 hp1
(Plasmid #89490)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 89490 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LMP
  • Backbone size w/o insert (bp) 7900
  • Total vector size (bp) 8016
  • Vector type
    Mammalian Expression, Mouse Targeting, Retroviral
  • Selectable markers
    Puromycin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hairpin against mouse/human AMPKalpha2
  • Insert Size (bp)
    116
  • Entrez Gene
    PRKAA2 (a.k.a. AMPK, AMPK2, AMPKa2, PRKAA)
  • Entrez Gene
    Prkaa2 (a.k.a. 2310008I11Rik, A830082D05, AMPKalpha2)
  • Promoter LMP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LMPseq1 or LMPseq2
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Ross Dickins in the Lowe lab, who also sold it to Open Biosystems

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LMP AMPK alpha2 hp1 was a gift from Russell Jones (Addgene plasmid # 89490 ; http://n2t.net/addgene:89490 ; RRID:Addgene_89490)
  • For your References section:

    AMPK is a negative regulator of the Warburg effect and suppresses tumor growth in vivo. Faubert B, Boily G, Izreig S, Griss T, Samborska B, Dong Z, Dupuy F, Chambers C, Fuerth BJ, Viollet B, Mamer OA, Avizonis D, DeBerardinis RJ, Siegel PM, Jones RG. Cell Metab. 2013 Jan 8;17(1):113-24. doi: 10.1016/j.cmet.2012.12.001. Epub 2012 Dec 27. 10.1016/j.cmet.2012.12.001 PubMed 23274086