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MSCV p2GM AMPK alpha2hp1 alpha1hp1
(Plasmid #89492)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89492 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV/LTRmiR30-PIGdeltaR1 (MSCV p2Gm)
  • Backbone size w/o insert (bp) 7894
  • Total vector size (bp) 8126
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    2 hairpins against mouse/human AMPKalpha1 and alpha2
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    232
  • Promoter U6 (The GFP-Puro is from a PGK promoter; the shRNA is from a U6 promoter)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV p2GM AMPK alpha2hp1 alpha1hp1 was a gift from Russell Jones (Addgene plasmid # 89492 ; http://n2t.net/addgene:89492 ; RRID:Addgene_89492)
  • For your References section:

    AMPK is a negative regulator of the Warburg effect and suppresses tumor growth in vivo. Faubert B, Boily G, Izreig S, Griss T, Samborska B, Dong Z, Dupuy F, Chambers C, Fuerth BJ, Viollet B, Mamer OA, Avizonis D, DeBerardinis RJ, Siegel PM, Jones RG. Cell Metab. 2013 Jan 8;17(1):113-24. doi: 10.1016/j.cmet.2012.12.001. Epub 2012 Dec 27. 10.1016/j.cmet.2012.12.001 PubMed 23274086