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pJK-caCas9-NatMX-Neut5L-Leu2 drive
(Plasmid #89578)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89578 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Leu2 gene drive
  • Species
    C. albicans
  • Insert Size (bp)
    1000

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK-caCas9-NatMX-Neut5L-Leu2 drive was a gift from George Church (Addgene plasmid # 89578 ; http://n2t.net/addgene:89578 ; RRID:Addgene_89578)
  • For your References section:

    A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans. Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE, Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins JJ. Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23. 10.1038/s41564-017-0043-0 PubMed 29062088