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Rc/CMV cyclin D2
(Plasmid #8958)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8958 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8958-D50 50 μg of DNA in Tris buffer $495
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DNA 8958-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    CCND2 (a.k.a. KIAK0002, MPPH3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length cyclin D2 cDNA excised from Bluescript SK+ with NcoI and XbaI and subcloned into Rc/CMV along with a HindIII to NcoI fragment of the cyclin D1 5'UTS that enhances expression. See author's map.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rc/CMV cyclin D2 was a gift from Philip Hinds (Addgene plasmid # 8958 ; http://n2t.net/addgene:8958 ; RRID:Addgene_8958)

  • For your References section:

    Multiple functions of D-type cyclins can antagonize pRb-mediated suppression of proliferation. Baker GL, Landis MW, Hinds PW. Cell Cycle. 2005 Feb . 4(2):330-8. 10.4161/cc.4.2.1485 PubMed 15684604
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