pKCAG-H2BmCherry-OptoJNKi
(Plasmid #89741)

• Purpose: Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11
• Depositing Lab: Michael Courtney
• Depositing Organization: University of Eastern Finland
  Located in Finland
• Publication: Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017.

Backbone

• Vector backbone: pmCherry-OptoJNKi
• Backbone manufacturer: Derived by our lab from pLuc-OptoJNKi and Cherry sequence
• Backbone size w/o insert (bp): 5240
• Total vector size (bp): 5680
• Vector type: Mammalian Expression ; Optogenetics
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OptoJNKi
• Alt name: AsLov2Jalpha (404-546)-JIP11
• Species: Synthetic; Avena Sativa and
• Insert Size (bp): 478
• GenBank ID: KY595943
• Promoter: CAG
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • Histone2B (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: DsRed_mCherry-fwd
• 3′ sequencing primer: fastbac reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We designed and assembled the insert from de novo synthesised sequences

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.

DNA (Catalog # 89741-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 89741-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.