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pmCherry-3xNLS-OptoJNKi-lsm
(Plasmid #89742)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89742 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 5240
  • Vector type
    Mammalian Expression ; Fluorescent protein
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-OptoJNKi lit-state mutant
  • Alt name
    3xNLS-AsLov2Jalpha (404-546)-I539E-JIP11
  • Species
    Synthetic; Avena Sativa and
  • Insert Size (bp)
    587
  • Mutation
    I539E mutation of AsLov2Jalpha
  • GenBank ID
    KY595936
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer DsRed_mCherry-fwd
  • 3′ sequencing primer fastbac reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We designed and assembled the insert from de novo synthesised sequences

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-3xNLS-OptoJNKi-lsm was a gift from Michael Courtney (Addgene plasmid # 89742 ; http://n2t.net/addgene:89742 ; RRID:Addgene_89742)
  • For your References section:

    A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Melero-Fernandez de Mera RM, Li LL, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ. Nat Commun. 2017 May 12;8:15017. doi: 10.1038/ncomms15017. 10.1038/ncomms15017 PubMed 28497795