Skip to main content
Addgene

pRK7-FLAG-TSC2
(Plasmid #8996)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8996 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK7
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSC2
  • Alt name
    tuberin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5400
  • Entrez Gene
    TSC2 (a.k.a. LAM, PPP1R160, TSC4)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK7-FLAG-TSC2 was a gift from John Blenis (Addgene plasmid # 8996 ; http://n2t.net/addgene:8996 ; RRID:Addgene_8996)
  • For your References section:

    Tuberous sclerosis complex-1 and -2 gene products function together to inhibit mammalian target of rapamycin (mTOR)-mediated downstream signaling. Tee AR, Fingar DC, Manning BD, Kwiatkowski DJ, Cantley LC, Blenis J. Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13571-6. 10.1073/pnas.202476899 PubMed 12271141