PurposeTo express GFP-tagged RvLEAM in mammalian cells transiently
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90035||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter CMV
/ Fusion Protein
- AcGFP1 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Insert species is Ramazzottius varieornatus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAcGFP1-N1-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90035)
For your References section:Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. Tanaka S, Tanaka J, Miwa Y, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Kubo T, Kunieda T. PLoS One. 2015 Feb 12;10(2):e0118272. eCollection 2015. DOI:10.1371/journal.pone.0118272 PubMed 25675104
Generated by Addgene from full sequence supplied by depositor.