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DD-Cas9 with filler sequence and Venus (EDCPV)
(Plasmid #90085)


Item Catalog # Description Quantity Price (USD)
Plasmid 90085 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • GenBank ID
  • Promoter EFS
  • Tags / Fusion Proteins
    • Destabilized Domain (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DD-Cas9 with filler sequence and Venus (EDCPV) was a gift from Raffaella Sordella (Addgene plasmid # 90085 ; ; RRID:Addgene_90085)
  • For your References section:

    Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization. Senturk S, Shirole NH, Nowak DG, Corbo V, Pal D, Vaughan A, Tuveson DA, Trotman LC, Kinney JB, Sordella R. Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370. 10.1038/ncomms14370 PubMed 28224990