Arf6 T27N- Venus in pcDNA3.1
(Plasmid #90464)

• Purpose: RhoA mediated cell migration
• Depositing Lab: Narasimhan Gautam
• Publication: Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.1021/acssynbio.9b00521.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6667
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Arf6 T27N
• Insert Size (bp): 528
• Entrez Gene: ARF6
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: T7 forward primer
• 3′ sequencing primer: BGH reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The entire Arf6 T27N-Venus can be cut out from pcDNA3.1 using EcoR1 and XbaI