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pLIX402 caPKA
(Plasmid #91702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91702 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pLIX402
  • Backbone manufacturer
    David Root (Addgene plasmid # 41394)
  • Backbone size w/o insert (bp) 9394
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKA
  • Alt name
    PRKACA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1055
  • Mutation
    constitutively active, H87Q and W196R that prevent sequestration by the regulatory subunit
  • GenBank ID
    NM_002730.3
  • Entrez Gene
    PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
  • Promoter TRE promoter, Tet ON

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX402 caPKA was a gift from Bob Weinberg (Addgene plasmid # 91702)

  • For your References section:

    Activation of PKA leads to mesenchymal-to-epithelial transition and loss of tumor-initiating ability. Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C, Reinhardt F, Tam WL, Weinberg RA. Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680. 10.1126/science.aad3680 PubMed 26941323
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