PurposePI(WT): Photo inhibitable (blue light sensitive)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91876||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7200
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesH. sapiens (human)
Insert Size (bp)1020
Entrez GeneVAV2 (a.k.a. VAV-2)
/ Fusion Protein
- mVenus (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer GAGATCAAGCAGAGGCTGAAG
- 3′ sequencing primer AAGTTCTTTTGCCGCCTCATC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-tetoff-mVenus-PICdc42 was a gift from Klaus Hahn (Addgene plasmid # 91876 ; http://n2t.net/addgene:91876 ; RRID:Addgene_91876)
For your References section:Engineering extrinsic disorder to control protein activity in living cells. Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM. Science. 2016 Dec 16;354(6318):1441-1444. 10.1126/science.aah3404 PubMed 27980211