pAAV-i53
(Plasmid #92170)

• Purpose: recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1
• Depositing Lab: Daniel Durocher
• Depositing Organization: Sinai Health System
  Located in Canada
• Publication: Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 2017 Nov 27.

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• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $34 USD for plasmid.
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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 4614
• Total vector size (bp): 4905
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: i53
• Alt name: synthetic engineered ubiquitin variant UbvG08 (i53)
• Species: Synthetic
• Insert Size (bp): 291
• Mutation: UbvG08 with I44A mutation and no terminal Glycines
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CGC AAA TGG GCG GTA GGC GTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Depositor Comments

bioRxiv (10.1101/060954)

DNA (Catalog # 92170-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 92170-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.