pENTR:3xHAgCYCLOPS-S154A
(Plasmid #98047)

• Purpose: 3xHA-gCYCLOPS-S154A for Gateway Cloning
• Depositing Lab: Martin Parniske
• Publication: Singh et al Cell Host Microbe. 2014 Feb 12;15(2):139-52. doi: 10.1016/j.chom.2014.01.011.

Backbone

• Vector backbone: pENTR-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2763
• Total vector size (bp): 6822
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYCLOPS
• Species: L. japonicus
• Insert Size (bp): 4059
• Mutation: serine 154 to alanine
• GenBank ID: AP009158.1
• Promoter: none
• Tag / Fusion Protein:
  • 3xHA-tag (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13_fwd GTAAAACGACGGCCAG
• 3′ sequencing primer: M13_rev CAGGAAACAGCTATGAC

Resource Information

• Supplemental Documents:
  • 98047-pENTR_3xHAgCYCLOPS-S154A.gbk
• A portion of this plasmid was derived from a plasmid made by: "CYCLOPS, a mediator of symbiotic intracellular accommodation." Yano et al., 2008

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry