PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98218||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5303
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameSynthetic construct Phobos_C128A gene
SpeciesSynthetic; Chlamydomonas reinhardtii
Insert Size (bp)927
MutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T159G, G163A, V242R, T246N, N258Q, E273S
- Promoter human synapsin
/ Fusion Protein
- Citrine (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GACTCAGCGCTGCCTCAGTCTG
- 3′ sequencing primer TGAACAGCTCCTCGCCCTTG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/early/2017/06/27/156422 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV2_hSyn_Phobos_CA_Citrine was a gift from Simon Wiegert (Addgene plasmid # 98218 ; http://n2t.net/addgene:98218 ; RRID:Addgene_98218)
For your References section:Anion-conducting channelrhodopsins with tuned spectra and modified kinetics engineered for optogenetic manipulation of behavior. Wietek J, Rodriguez-Rozada S, Tutas J, Tenedini F, Grimm C, Oertner TG, Soba P, Hegemann P, Wiegert JS. Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.1038/s41598-017-14330-y. 10.1038/s41598-017-14330-y [pii] PubMed 29097684