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pCS2+ gfp-zASC
(Plasmid #98703)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98703 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 5430
  • Modifications to backbone
    Multiple cloning site was shortened during insertion of the construct
  • Vector type
    Mammalian Expression ; Zebrafish/Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Apoptosis associated speck like protein containing CARD
  • Alt name
    ASC
  • Alt name
    PYCARD
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1357
  • GenBank ID
    NC_007127.6
  • Entrez Gene
    pycard (a.k.a. ASC, asc1, zAsc, zgc:109874)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: This plasmid has an I78N mutation compared to the depositor's sequence and reference sequence. The functional impact of this mutation has not been verified.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ gfp-zASC was a gift from Anna Huttenlocher (Addgene plasmid # 98703 ; http://n2t.net/addgene:98703 ; RRID:Addgene_98703)
  • For your References section:

    Macrophages mediate flagellin induced inflammasome activation and host defense in zebrafish. Vincent WJ, Freisinger CM, Lam PY, Huttenlocher A, Sauer JD. Cell Microbiol. 2016 Apr;18(4):591-604. doi: 10.1111/cmi.12536. Epub 2015 Nov 4. 10.1111/cmi.12536 PubMed 26468080