Casp1
(Plasmid #98849)

• Purpose: Expresses murine Casp1 in mammalian cells
• Depositing Lab: Peter Keyel
• Publication: Shi et al Front Immunol. 2017 May 8;8:522. doi: 10.3389/fimmu.2017.00522. eCollection 2017.

Backbone

• Vector backbone: pCFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 7200
• Modifications to backbone: CFP removed during cloning process
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Casp1
• Alt name: Caspase-1
• Alt name: ICE
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• GenBank ID: BC008152
• Entrez Gene: Casp1 (a.k.a. ICE, Il1bc)
• Promoter: CMV
• Tag / Fusion Protein:
  • No fusion proteins or tags

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV forward (Genewiz Universal)
• 3′ sequencing primer: SV40 poly A reverse (Genewiz Universal)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Casp1 cloned by PCR initially from Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry