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pK7WG_ASX
(Plasmid #98889)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98889 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pK7WG
  • Backbone manufacturer
    VIB
  • Backbone size (bp) 10167
  • Modifications to backbone
    Release of T35S–AttR2–ccdB–AttR1 cassette from the vector backbone. Introduction of I-SceI–A0–HindIII–ccdB–HindIII–B0–I-SceI cassette, to add A0 and B0 AssemblX compatible homology regions with flanking restriction enzymes recognition sites and the ccdB gene. pK7WG_ASX is compatible with AssemblX cloning system.
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    /
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK7WG_ASX was a gift from Kristina Gruden (Addgene plasmid # 98889 ; http://n2t.net/addgene:98889 ; RRID:Addgene_98889)
  • For your References section:

    Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants. Lukan T, Machens F, Coll A, Baebler S, Messerschmidt K, Gruden K. PLoS One. 2018 Jan 4;13(1):e0190526. doi: 10.1371/journal.pone.0190526. eCollection 2018. 10.1371/journal.pone.0190526 PubMed 29300787