pAAV-RepCap8-v3 (add p5 promoter 3' of Cap gene)
(Plasmid
#99290)
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PurposeRepCap plasmid for AAV production, serotype 8
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC19 with additional elements
- Total vector size (bp) 7271
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSerotype 8 Cap
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Speciesparvovirus
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Insert Size (bp)2255
Gene/Insert 2
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Gene/Insert nameRep
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Speciesparvovirus
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Insert Size (bp)1865
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Modified pRC8: addition of p5' promoter in 3' (downstream of Cap gene)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-RepCap8-v3 (add p5 promoter 3' of Cap gene) was a gift from Melina Fan (Addgene plasmid # 99290)