pFastBac-ATG101-ATG13 HORMA
(Plasmid #99332)

• Purpose: Mammalian expression of one subcomplex from the Human ULK1 complex
• Depositing Lab: James Hurley
• Publication: Qi et al Structure. 2015 Oct 6;23(10):1848-57. doi: 10.1016/j.str.2015.07.011. Epub 2015 Aug 20.

Backbone

• Vector backbone: pFastBac-Dual
• Backbone manufacturer: Invitrogen
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ATG101
• Species: H. sapiens (human)
• GenBank ID: NP_068753.2
• Entrez Gene: ATG101 (a.k.a. C12orf44)
• Promoter: p10

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: p10_Fwd: 5'- TACGGACCTTTAATTCAACCC -3'
• 3′ sequencing primer: p10_rev: 5'- TTCCGGTATTGTCTCCTTCCGT

Gene/Insert 2

• Gene/Insert name: ATG13
• Species: H. sapiens (human)
• GenBank ID: NP_001136145.1
• Entrez Gene: ATG13 (a.k.a. KIAA0652, PARATARG8)
• Promoter: pH
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pH_Fwd:5'- TCCGGATTATTCATACCGTC -3'
• 3′ sequencing primer: pH_rev:5'- TGTGGTATGGCTGATTATGATC -3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a dual insertion: ATG101 in p10 cassette; GST-TEVsite-ATG13 (12-200) in pH cassette.