pET-ZZ(1700-1751)
(Plasmid
#99334)
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PurposeBacterial expression of mouse CBP ZZ domain
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 5605
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCBP ZZ domain
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SpeciesM. musculus (mouse)
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Insert Size (bp)162
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Entrez GeneCrebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-ZZ(1700-1751) was a gift from Peter Wright (Addgene plasmid # 99334 ; http://n2t.net/addgene:99334 ; RRID:Addgene_99334) -
For your References section:
ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module. Legge GB, Martinez-Yamout MA, Hambly DM, Trinh T, Lee BM, Dyson HJ, Wright PE. J Mol Biol. 2004 Oct 29;343(4):1081-93. 10.1016/j.jmb.2004.08.087 PubMed 15476823
