PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99531||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)2508
Entrez GeneAFB2 (a.k.a. AT3G26810, auxin signaling F-box 2)
- Promoter ADH1
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site PstI (unknown if destroyed)
A portion of this plasmid was derived from a plasmid made byThe AFB2 gene was a kind gift from Jennifer L. Nemhauser (University of Washington, Seattle, USA).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Digest vector with EcoRV for integration into TRP1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YIp204-PADH1-AFB2 was a gift from Helle Ulrich (Addgene plasmid # 99531 ; http://n2t.net/addgene:99531 ; RRID:Addgene_99531)