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Bryan Berger Lab Plasmids


The Bryan Berger Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Identifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. Su PC, Berger BW J Biol Chem. 2012 Jul 22.
High-Yield membraneprotein expression fromE. coliusing an engineered outer membrane protein F (OmpF) fusion. Su PC, Si W, Baker DL, Berger BW Protein Sci. 2013 Jan 24. doi: 10.1002/pro.2224.
A Novel Assay for Assessing Juxtamembrane and Transmembrane Domain Interactions Important for Receptor Heterodimerization. Su PC, Berger BW J Mol Biol. 2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 10.1016/j.jmb.2013.07.022.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
39547pAraTMBacterial ExpressionIdentifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. J Biol Chem. 2012 Jul 22. pTrc99 Add to Cart
39548pAraGFPeGFPBacterial ExpressionIdentifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. J Biol Chem. 2012 Jul 22. pBAD18 Add to Cart
39549pAraTM-2BTMCYwtIntegrin alpha2B TM-CYTO (Synthetic)Bacterial ExpressionIdentifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. J Biol Chem. 2012 Jul 22. pTrc99 Add to Cart
39550pAraTM-2BTMCYL980AIntegrin alpha2B L980A TM-CYTO (Synthetic)Bacterial ExpressionIdentifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. J Biol Chem. 2012 Jul 22. pTrc99 Add to Cart
42606pOmpFBacterial ExpressionHigh-Yield membraneprotein expression fromE. coliusing an engineered outer membrane protein F (OmpF) fusion. Protein Sci. 2013 Jan 24. doi: 10.1002/pro.2224. pET28 Add to Cart
42894MM39Identifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. J Biol Chem. 2012 Jul 22. genome Add to Cart
47514pAraTMwtIntegrin alpha2B L980A TM-CYTO (Synthetic)Bacterial ExpressionA Novel Assay for Assessing Juxtamembrane and Transmembrane Domain Interactions Important for Receptor Heterodimerization. J Mol Biol. 2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 10.1016/j.jmb.2013.07.022. Contains A2B L980A TMCY mutant, expresses wt AraC for heterodimer competition assay pTrc99 Add to Cart
47515pAraTMDNIntegrin alpha2B L980A TM-CYTO (Synthetic)Bacterial ExpressionA Novel Assay for Assessing Juxtamembrane and Transmembrane Domain Interactions Important for Receptor Heterodimerization. J Mol Biol. 2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 10.1016/j.jmb.2013.07.022. Contains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assay pRSF Add to Cart
47516pAraGFPCDFGFP (Other)Bacterial ExpressionA Novel Assay for Assessing Juxtamembrane and Transmembrane Domain Interactions Important for Receptor Heterodimerization. J Mol Biol. 2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 10.1016/j.jmb.2013.07.022. Contains PBAD::GFP reporter, used for heterodimer competition assay pCDF Add to Cart