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Jay Hesselberth Lab Plasmids


The Jay Hesselberth Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Capture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. Schutz K, Hesselberth JR, Fields S RNA. 2010 Mar;16(3):621-31. Epub 2010 Jan 14.
Global analysis of RNA cleavage by 5'-hydroxyl RNA sequencing. Peach SE, York K, Hesselberth JR Nucleic Acids Res. 2015 May 22. pii: gkv536.
pDONR221-10X_tRNA (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
32242pET28a-At-TRLA. thaliana tRNA ligase (Arabidopsis thaliana)Bacterial ExpressionCapture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. RNA. 2010 Mar;16(3):621-31. Epub 2010 Jan 14. pET28a RNL AT1G07910, ARABIDOPSIS THALIANA RNA LIGASE, ATRNL, RNAligase, T6D22.1, T6D22_1, TRNA LIGASE Add to Cart
42571pDEST17-Tpt1Tpt1 (Saccharomyces cerevisiae)Bacterial ExpressionCapture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. RNA. 2010 Mar;16(3):621-31. Epub 2010 Jan 14. pDEST17 Add to Cart
51282pET-53-RtcBE. coli RtcB (Other)Bacterial ExpressionGlobal analysis of RNA cleavage by 5'-hydroxyl RNA sequencing. Nucleic Acids Res. 2015 May 22. pii: gkv536. E. coli expression vector for E. coli RtcB RNA ligase pET-53-DEST Add to Cart
70123pDONR221-10X-tRNA10X-tRNA block (Saccharomyces cerevisiae) pDONR221-10X_tRNA (unpublished) Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs pDONR221 Add to Cart
70124pAG424-ccdBYeast Expression pDONR221-10X_tRNA (unpublished) Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed) pRS424-GPD-ccdB Add to Cart
70125pAG424-10X-tRNA10X-tRNA block (Saccharomyces cerevisiae)Yeast Expression pDONR221-10X_tRNA (unpublished) Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns pAG424-ccdB Add to Cart