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Masato Ohtsuka Lab Plasmids


The Masato Ohtsuka Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
Assessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato M, Ohtsuka M PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015.
Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83.
Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination. Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H Transgenic Res. 2013 Aug;22(4):873-5. doi: 10.1007/s11248-013-9703-x. Epub 2013 Mar 26.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
62657pAYCiCreMammalian ExpressionOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62658pBCWPhiC31Mammalian ExpressionOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62659pBCVFLPoMammalian ExpressionOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62660pBDTeGFP, neomycinMouse TargetingOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Targeting vector for PITT landing pad knock-in into the Rosa26 locus pBS Add to Cart
62661pBDUeGFP, neomycinMouse TargetingOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Targeting vector for PITT landing pad knock-in into the Rosa26 locus pBS Add to Cart
62662pBERtdTomato, hygromycinCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for tdTomato knock-in via landing pad pSMART-kan Add to Cart
62663pBDRtdTomatoCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for tdTomato knock-in via landing pad pSMART-kan Add to Cart
62664pBGVmCFPCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for mCFP knock-in via landing pad pSMART-kan Add to Cart
62665pBGWmCitrineCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for mCitrine knock-in via landing pad pSMART-kan Add to Cart
62666pBGXDreCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for Dre knock-in via landing pad pSMART-kan Add to Cart
62667pBGTiRFPCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for iRFP knock-in via landing pad pSMART-kan Add to Cart
62668pBFBtdTomatoCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for tdTomato knock-in via landing pad pSMART-kan Add to Cart
62669pBEZtdTomatoCre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for tdTomato knock-in via landing pad pSMART-kan Add to Cart
62670pBBKPhiC31Mammalian Expression, Synthetic BiologyOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Vector for PhiC31o mRNA synthesis pcDNA3.1 Add to Cart
62671pBGOFTH1Mammalian Expression, Cre/LoxOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Donor vector for mFTH1 knock-in via landing pad pSMART-kan Add to Cart
62672pBBJFLPoOne-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. Vector for FLPo mRNA synthesis pcDNA3.1 Add to Cart
62680pARA (pKD-G1)tdTomato/amiR-eGFP123Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62681pARB (pKD-G2)tdTomato/amiR-eGFP419Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62682pARC (pKD-G3)amiR-eGFP123Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62683pARD (pKD-G4)amiR-eGFP419Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62684pATG (pKD-G5)tdTomato/amiR-eGFP123/amiR-eGFP419Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62685pATH (pKD-G6)tdTomato/amiR-eGFP419/amiR-eGFP123Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62686pATI (pKD-G7)amiR-eGFP123/amiR-eGFP419Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62687pATJ (pKD-G8)amiR-eGFP419/amiR-eGFP123Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62688pATK (pKD-G9)tdTomato/amiR-eGFP123/amiR-eGFP419Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62689pATL (pKD-G10)amiR-eGFP419/amiR-eGFP123Mammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62690pATM (pKD-G11)amiR-eGFP123/amiR-eGFP419/tdTomatoMammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62691pATN (pKD-G12)amiR-eGFP419/amiR-eGFP123/tdTomatoMammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62707pAQI (pT-1)tdTomatoMammalian ExpressionAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62708pART (pT-2)tdTomatoMammalian ExpressionAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer pUC119 Add to Cart
62709pAYP (pDre)DreMammalian ExpressionAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer pCAGGS Add to Cart
62710pAWVtdTomato/amiR-eGFP123/amiR-eGFP419/FLPeMammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Donor vector for amiR-eGFP knock-in via landing pad pBR322 Add to Cart
62713pAWKamiR-eGFP123/amiR-eGFP419/FLPeMammalian Expression, RNAiAssessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. Donor vector for amiR-eGFP knock-in via landing pad pBR322 Add to Cart
65795pBBIiCreMammalian Expression, Cre/LoxImprovement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination. Transgenic Res. 2013 Aug;22(4):873-5. doi: 10.1007/s11248-013-9703-x. Epub 2013 Mar 26. Vector for iCre mRNA synthesis pcDNA3.1 Add to Cart
65796pBGKCas9Mammalian Expression, CRISPRMouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. Vector for Cas9 mRNA synthesis pcDNA3.1 Add to Cart