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Silvestro Conticello Lab Plasmids


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Title Authors Publication
An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
rosa26 knock-in plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
54321pBS SK mCherryROSAbsrmCherry and BlasticidinS-resistance (Other)Mammalian Expression, Mouse Targeting, CRISPR, TALENAn efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR. pBS SK(+) Add to Cart
54322pBS SK mCherryROSAegfpmCherry and EGFP (Other)Mammalian Expression, Mouse Targeting, CRISPR, TALENAn efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR. pBS SK(+) Add to Cart
54323pCAGGS-ROSAfTALEN for the murine ROSA26 locus (Other)Mammalian Expression, Mouse Targeting, TALEN rosa26 knock-in plasmids (unpublished) TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid pCAGGS-TAL-NC2 Add to Cart
54324pCAGGS-ROSArTALEN for the murine ROSA26 locus (Other)Mammalian Expression, Mouse Targeting, TALEN rosa26 knock-in plasmids (unpublished) TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid pCAGGS-TAL-NC2 Add to Cart
66950pBSR (mCherry-surrogateTarget-bsr)blasticidin (Other)Mammalian Expression, Mouse Targeting, CRISPR, TALENAn efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR. pBS SK Add to Cart